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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so might contribute to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but specific nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible final results are...
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This guideline outlines further achievable optimization of cellular and immunoassay parameters to be sure the very best success are...
Antibodies sure to the blocking peptide no longer bind for the epitope over the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.
Target Info Cyclin E belongs to the highly conserved cyclin relatives, whose customers are characterised by a spectacular periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which add to your temporal coordination of every mitotic event. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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